High-throughput screening of SSH cDNA libraries using DNA microarray data

Description

SSHscreen was developed as a result of the analysis method described in Van den Berg et al. (2004). The laboratory protocol and first version SSHscreen 1.0.1 implementing more sophisticated normalization and statistical analysis steps (using the limma package) is described in Berger et al. (2007). The current version of SSHscreen 2.0.1 is described in Coetzer et al. (2010). We recommend the use of >=R-2.2.0 for this package.

Note: The ACGT Microarray Facility provides a service for gene discovery projects using SSHscreen analysis and SSHdb management of sequences as described in Coetzer et al. (2010). Please contact Nicky Olivier.

SSHdb is a web-accessible database for managing clone sequences. It combines the SSHscreen data with sequence annotations derived from BLAST and BLAST2GO. Please register at http://sshdb.bi.up.ac.za

SSHscreen_2.0.1

Download the latest version of SSHscreen

Example of an ER3 vs inverse ER2 analysis (forward and reverse libraries)

Download demonstration data:


Screen Plot of ER3 vs inverse ER2 analysis:

SSHscreen_2.0.0

Download the previous version of SSHscreen:

Example of an ER3 vs inverse ER2 analysis

See Berger et al. (2007) for more detail.

Download demonstration data:


Screen Plot of ER3 vs inverse ER2 analysis:

SSHscreen_1.0.1

Download the previous version of SSHScreen:

Example of an ER1vsER2 analysis:

Download demonstration data:


Screen Plot of ER3 vs inverse ER2 analysis:

Author(s)

Wiesner Vos, Dave Berger, Nanette Coetzer (nanette.coetzer@gmail.com)

References:

Contact information

For more information about SSHscreen software, contact Prof Dave Berger.